The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
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Facts-enriched characterization of mobile populations. Morphological facts from illustrations or photos can insert to your richness of flow cytometry facts. As an example, the determine displays an in any other case standard apoptosis assay using Annexin V and PI, introducing cell imaging to characterize cells in Just about every populace to reveal morphologically distinctive options. These insights couldn't are attained from multiplex staining by yourself.
As samples are obtained over the Attune CytPix Flow Cytometer, the significant-speed brightfield camera captures and shops pictures of detected activities at a price as many as 6,000 photos per next, based on the flow price and impression size. For larger adaptability, Attune Cytometric Software program helps you to alter the impression capture frequency as required and select a certain gate for picture capture, if required.
Combine acoustic concentrating technology with higher-parameter Assessment for adaptable spectral unmixing and standard workflows.
Find out new insights into your experimental info by visualizing populations utilizing mixed fluorescence and morphology knowledge. Our application interface wizards enable downsampling, concatenation (merged files) and dimensionality applications which might be created in to the application for an intuitive, streamlined information Investigation practical experience.
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On top of that, it really is illuminating to determine the quantity of doublets slip as a result of common singlet gates, and terrific to confirm the status of surprising double-constructive situations in my Assessment."
Pair your Attune flow cytometer with an autosampler for amplified workflow effectiveness, and insert robotic integration for maximum throughput.
Steady picture high quality even at substantial throughput Acoustic focusing along with a higher-pace camera Mix to graphic these T cells persistently at minimal or significant flow prices. Easily regulate concentrate and digital camera options to meet experimental prerequisites.
Early indications of this contamination had at first been dismissed as particles. Backgating to find new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also enables you to use morphological functions to discover exciting subpopulations that may not be evident from flow cytometry knowledge on your own.
Users can perspective event visuals in authentic-time to verify sample quality, enhance protocols, and document the morphology of each mobile population discovered for Evaluation. Imaging capture also Rewards from acoustic concentrating, which really helps to placement the cells to ensure that a sharp, centered graphic is attained.
Audio waves help exact mobile alignment, leading to Outstanding knowledge excellent and ~10X a lot quicker sample flow charges.
We Formerly shown the strength of imaging Auto-T/Ramos cell interactions. Permit’s take a look at just the population of biggest curiosity, the double good occasions, to learn more. We are able to now use extended morphological parameters (in this article, circularity and skewness of intensity) to even more take a look at the options of such populations and refine gating on these gatherings, growing info robustness.
Developed-in dimensionality reduction resources greater resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm envisioned population resolution in lysed full human blood. Person can check out situations from Each and every cluster for visual confirmation of cell phenotypes.
Versatile payment prepare from Agilent Economical Methods permit you to obtain the essential instrumentation you'll need for immediate project, or to expand your lab.
At the center of each Attune Flow Cytometer lies our revolutionary acoustic concentrating technological innovation, which utilizes seem waves to meticulously align cells in the flow mobile. This innovative tactic surpasses common hydrodynamic methods, resulting in more quickly processing speeds, Excellent information quality, and greater sample throughput.
The Attune Xenith cytometer is a high-parameter spectral analyzer, supplying both spectral unmixing and traditional flow abilities for comprehensive and adaptable cell Investigation.
The Attune CytPix Flow Cytometer permits users to visualize samples all through acquisition, verify the accuracy of gating tactics, and assistance final results with reliable documentation for here publication applications.
Here, we show that by gating tactics using graphic-centered quantitative parameters can distinguish interacting cells from coincident events to more accurately assess interacting cells.